Issue N# 3 - 2009
Cytokines profile in cystic fibrosis patients with nasal polyps
Authors : Nunes FB, Becker CG, Becker HMG, Araujo RN, Guimarães RES. (Belo Horizonte)
Ref. : Rev Laryngol Otol Rhinol. 2009;130,3:163-167.
Article published in english
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Nasal polyposis often complicates the progress of patients with cystic fibrosis and there has been little study about the importance of cytokines in the polyps of such individuals. Objective: To assess RNAm expression for interleukins 4, 5, 6, 8, GM-CSF and IFN-g by RT-PCR in eosinophilic and non-eosinophilic polyps of patients with cystic fibrosis. Material and method: A total of 124 persons were evaluated, of which thirteen patients with cystic fibrosis and nasosinusal polyps were selected – three were eosinophilic and ten were non-eosinophilic. The control group was composed of eleven individuals with normal otorhinolaryngological exam and the mean age was 18 years (3-57). The middle turbinate mucosa and nasal polyps were biopsied from the control group and the cystic fibrosis group respectively, and these were analyzed with RT-PCR The middle turbinate mucosa was biopsied in the control group and in the cystic fibrosis group polyps that was analyzed to RT-PCR. The polyps of cystic fibrosis patients were also further anaylsed for subjected to a second biopsy in order to determine the percentage of eosinophils. IL-4, IL-5, IL-6, IL-8, IFN-g and GM-CSF transcriptions were analyzed. Results: There was no difference in IL-5, IL-8 and GM-CSF when compared to the eosinophilic, non-eosinophilic and control groups (p> 0.05). When compared to the eosinophilic and non-eosinophilic groups, higher IL-4 and IL-6 values (p= 0.01 and p= 0.01 respectively) were observed. When analyzed separately with the control group, IL-4 (p= 0.01) expression was higher in the eosinophilic group, while IFN-g (p= 0.03) was lower in the non-eosinophilic group. IL-5, IL-8, GM-CSF are non-specific cytokines present in the nasosinusal sinonasal polyps of cystic fibrosis patients. IL-4 and IL-6 are important mediators in the eosinophilic sinonasal polyps, while low IFN-g may be related to lower eosinophils in non-eosinophilic polyps.
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